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DENN / MADD Antibody

LS-C816648
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetMADD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DENN / MADD Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8567
  • Target name
    MADD
  • Target description
    MAP kinase activating death domain
  • Target synonyms
    DEEAH, DENN, IG20, NEDDISH, RAB3GEP, RabGEF, MAP kinase-activating death domain protein, Insuloma-Glucagonoma protein 20, Rab3 GDP/GTP exchange factor, differentially expressed in normal and neoplastic cells, insulinoma glucagonoma clone 20, rab3 GDP/GTP exchange protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MADD using anti-MADD antibody (A02030-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADD antigen affinity purified polyclonal antibody (Catalog # A02030-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADD at approximately 240 kDa. The expected band size for MADD is at 183 kDa.
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