Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2(GTX102508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102508/GTX102508_43810_20201023_IHC-P_w_23060100_755.webp "Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX102508/GTX102508_43810_20210924_ICC_IF_w_23060100_885.webp "Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102508/GTX102508_43810_20201008_WB_TPM_watermark_w_23060100_669.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane and cytoplasm in mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102508/GTX102508_40114_20170817_IHC-P_M_w_23060100_507.webp "Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane and cytoplasm in mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102508/GTX102508_43810_20200904_WB_M_heart_w_23060100_352.webp "Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2(GTX102508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Desmoglein 2 antibody [N1N2], N-term
GTX102508
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetDSG2
Overview
- SupplierGeneTex
- Product NameDesmoglein 2 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.36 mg/ml
- ConjugateUnconjugated
- Gene ID1829
- Target nameDSG2
- Target descriptiondesmoglein 2
- Target synonymsCDHF5, HDGC, desmoglein-2, cadherin family member 5
- HostRabbit
- IsotypeIgG
- Protein IDQ14126
- Protein NameDesmoglein-2
- Scientific DescriptionDesmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Liu Y, Chidgey M, Yang VW, et al. Krüppel-like factor 5 is essential for maintenance of barrier function in mouse colon. Am J Physiol Gastrointest Liver Physiol. 2017,313(5):G478-G491. doi: 10.1152/ajpgi.00172.2017Read this paper
Datasheet
Related products
Product group Antibodies
Anti-desmoglein-2 [6D8]Ab01658-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetDSG2
- SizePrice
Product group Antibodies
Anti-Desmoglein 2 / DSG2 Antibody188-11060
ReactivityHuman
TargetDSG2
- SizePrice
Product group Antibodies
Anti-Desmoglein 2/DSG2 Antibody Picoband(r)A02035-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDSG2
- SizePrice
![Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [HL2036] (GTX637937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637937/GTX637937_T-44879_20221202_WB_M_heart_22120719_293.webp)
Product group Antibodies
Desmoglein 2 antibody [HL2036]GTX637937
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetDSG2
- SizePrice
Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityPorcine
TargetDSG2
- SizePrice
Product group Antibodies
References
Desmoglein 2 Polyclonal AntibodyBS-10152R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetDSG2
- SizePrice
Product group Antibodies
Anti-DSG2 AntibodyA101469
ApplicationsWestern Blot, ELISA
ReactivityHuman
- SizePrice
Product group Antibodies
Anti-DSG2 AntibodyHPA004896
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDSG2
- SizePrice