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Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.

Desmoglein 2 Polyclonal Antibody

BS-10152R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDSG2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Desmoglein 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1829
  • Target name
    DSG2
  • Target description
    desmoglein 2
  • Target synonyms
    cadherin family member 5; CDHF5; desmoglein-2; HDGC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14126
  • Protein Name
    Desmoglein-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague-Dawley rats. Xue Y et al., 2019 Nov, Food Chem Toxicol
    Read more

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Figure 2. IHC analysis of DSG2 using anti-DSG2 antibody (A02035). DSG2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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