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Desmoglein 2/DSG2 Antibody

10-2298
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    Desmoglein 2/DSG2 Antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
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Figure 2. IHC analysis of DSG2 using anti-DSG2 antibody (A02035). DSG2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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