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DHX15 Antibody

LS-C816253
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetDHX15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DHX15 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1665
  • Target name
    DHX15
  • Target description
    DEAH-box helicase 15
  • Target synonyms
    ATP-dependent RNA helicase #46; DBP1; DDX15; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15; DEAD/H box-15; DEAH (Asp-Glu-Ala-His) box helicase 15; DEAH (Asp-Glu-Ala-His) box polypeptide 15; DEAH box protein 15; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PRPF43; PrPp43p; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; RNA helicase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human U251 whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX15/prp43 antigen affinity purified polyclonal antibody (Catalog # A08140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX15/prp43 at approximately 91 kDa. The expected band size for DHX15/prp43 is at 91 kDa.
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