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DIAPH3 Antibody

CAB10351
Assay Genie
TargetSTAR
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    DIAPH3 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6770
  • Target name
    STAR
  • Target description
    steroidogenic acute regulatory protein
  • Target synonyms
    STARD1, steroidogenic acute regulatory protein, mitochondrial, START domain containing 1, START domain-containing protein 1, StAR related lipid transfer (START) domain containing 1, cholesterol trafficker, mitochondrial steroid acute regulatory protein, steroid acute regulatory protein, steroidogenic acute regulator, testis secretory sperm-binding protein Li 241mP
  • Host
    Rabbit
  • Protein IDP49675
  • Protein Name
    Steroidogenic acute regulatory protein, mitochondrial
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of StAR using anti-StAR antibody (A00051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
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