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WB analysis of human heart lysate using GTX41187 DKK2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human heart lysate using GTX41187 DKK2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human heart lysate using GTX41187 DKK2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer

DKK2 antibody, C-term

GTX41187
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetDKK2
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Overview

  • Supplier
    GeneTex
  • Product Name
    DKK2 antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27123
  • Target name
    DKK2
  • Target description
    dickkopf WNT signaling pathway inhibitor 2
  • Target synonyms
    dickkopf 2 homolog; dickkopf homolog 2; dickkopf-2; dickkopf-related protein 2; DKK-2; hDkk-2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UBU2
  • Protein Name
    Dickkopf-related protein 2
  • Scientific Description
    This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DKK2 using anti-DKK2 antibody (PB9551). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK2 antigen affinity purified polyclonal antibody (Catalog # PB9551) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK2 at approximately 28 kDa. The expected band size for DKK2 is at 28 kDa.
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