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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: DKK2 antibody at 3.9microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: DKK2 antibody at 3.9microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: DKK2 antibody at 3.9microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 32 kDa

DKK2 Antibody

CSB-PA08279A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDKK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    DKK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27123
  • Target name
    DKK2
  • Target description
    dickkopf WNT signaling pathway inhibitor 2
  • Target synonyms
    dickkopf 2 homolog; dickkopf homolog 2; dickkopf-2; dickkopf-related protein 2; DKK-2; hDkk-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBU2
  • Protein Name
    Dickkopf-related protein 2
  • Scientific Description
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Human Brain lysates; Lane 2: Mouse Heart lysates probed with DKK2 Monoclonal Antibody, Unconjugated (bsm-51690M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of DKK2 using anti-DKK2 antibody (PB9551). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK2 antigen affinity purified polyclonal antibody (Catalog # PB9551) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK2 at approximately 28 kDa. The expected band size for DKK2 is at 28 kDa.
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