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Lane 1: Human Brain lysates; Lane 2: Mouse Heart lysates probed with DKK2 Monoclonal Antibody, Unconjugated (bsm-51690M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Brain lysates; Lane 2: Mouse Heart lysates probed with DKK2 Monoclonal Antibody, Unconjugated (bsm-51690M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Brain lysates; Lane 2: Mouse Heart lysates probed with DKK2 Monoclonal Antibody, Unconjugated (bsm-51690M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

DKK2 Monoclonal Antibody

BSM-51690M
Bioss Antibodies
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetDKK2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DKK2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:500-1000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    J3F5
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID27123
  • Target name
    DKK2
  • Target description
    dickkopf WNT signaling pathway inhibitor 2
  • Target synonyms
    dickkopf 2 homolog; dickkopf homolog 2; dickkopf-2; dickkopf-related protein 2; DKK-2; hDkk-2
  • Host
    Mouse
  • Protein IDQ9UBU2
  • Protein Name
    Dickkopf-related protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DKK2 using anti-DKK2 antibody (PB9551). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK2 antigen affinity purified polyclonal antibody (Catalog # PB9551) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK2 at approximately 28 kDa. The expected band size for DKK2 is at 28 kDa.
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