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Immunohistochemistry analysis of human kidney tissue using CSB-PA614264LA01HU at dilution of 1:100
Immunohistochemistry analysis of human kidney tissue using CSB-PA614264LA01HU at dilution of 1:100
Immunohistochemistry analysis of human kidney tissue using CSB-PA614264LA01HU at dilution of 1:100

DLG1 Antibody

CSB-PA614264LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDLG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DLG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1739
  • Target name
    DLG1
  • Target description
    discs large MAGUK scaffold protein 1
  • Target synonyms
    discs large homolog 1, scribble cell polarity complex component; disks large homolog 1; dJ1061C18.1.1; DLGH1; hdlg; presynaptic protein SAP97; SAP97; SAP-97; synapse-associated protein 97
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12959
  • Protein Name
    Disks large homolog 1
  • Scientific Description
    Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAP97 using anti-SAP97 antibody (PB9552). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: Mouse Lung Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MM231 Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug, Lane 6: A549 Whole Cell Lysate at 40ug, Lane 7: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP97 antigen affinity purified polyclonal antibody (Catalog # PB9552) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP97 at approximately 130 kDa. The expected band size for SAP97 is at 130 kDa.
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