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ProductsBack/DLG1 / SAP97 Antibody

DLG1 / SAP97 Antibody

LS-C747534
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetDLG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DLG1 / SAP97 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 87-103kDa, while the observed MW by Western blot was 120kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 87-103kDa, while the observed MW by Western blot was 120kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1739
  • Target name
    DLG1
  • Target description
    discs large MAGUK scaffold protein 1
  • Target synonyms
    DLGH1, SAP-97, SAP97, hdlg, disks large homolog 1, dJ1061C18.1.1, discs large homolog 1, scribble cell polarity complex component, presynaptic protein SAP97, synapse-associated protein 97
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAP97 using anti-SAP97 antibody (PB9552). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: Mouse Lung Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MM231 Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug, Lane 6: A549 Whole Cell Lysate at 40ug, Lane 7: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP97 antigen affinity purified polyclonal antibody (Catalog # PB9552) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP97 at approximately 130 kDa. The expected band size for SAP97 is at 130 kDa.
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