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Dlg4 Recombinant Antibody

CAC12531
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetDLG4
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Overview

  • Supplier
    Biomatik
  • Product Name
    Dlg4 Recombinant Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1H4
  • Conjugate
    Unconjugated
  • Gene ID1742
  • Target name
    DLG4
  • Target description
    discs large MAGUK scaffold protein 4
  • Target synonyms
    discs large homolog 4; disks large homolog 4; MRD62; post-synaptic density protein 95; PSD95; SAP90; SAP-90; synapse-associated protein 90; Tax interaction protein 15
  • Isotype
    IgG
  • Protein IDP78352
  • Protein Name
    Disks large homolog 4
  • Scientific Description
    The Dlg4 Recombinant Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (A02128). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95/DLG4 antigen affinity purified polyclonal antibody (Catalog # A02128) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95/DLG4 at approximately 99KD. The expected band size for PSD95/DLG4 is at 99KD.
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