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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

PSD95 Polyclonal Antibody

BS-0179R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDLG4
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Overview

  • Supplier
    Bioss
  • Product Name
    PSD95 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1742
  • Target name
    DLG4
  • Target description
    discs large MAGUK scaffold protein 4
  • Target synonyms
    MRD62, PSD95, SAP-90, SAP90, disks large homolog 4, Tax interaction protein 15, discs large homolog 4, post-synaptic density protein 95, synapse-associated protein 90
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78352
  • Protein Name
    Disks large homolog 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu B, Liu J, Zhang J, et al. Effects of autophagy on synaptic-plasticity-related protein expression in the hippocampus CA1 of a rat model of vascular dementia. Neurosci Lett. 2019,707:134312. doi: 10.1016/j.neulet.2019.134312
    Read this paper
  • Ku T, Chen M, Li B, et al. Synergistic effects of particulate matter (PM(2.5)) and sulfur dioxide (SO(2)) on neurodegeneration via the microRNA-mediated regulation of tau phosphorylation. Toxicol Res (Camb). 2017,6(1):7-16. doi: 10.1039/c6tx00314a
    Read this paper
  • Ku T, Li B, Gao R, et al. NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM(2.5) aspiration. Part Fibre Toxicol. 2017,14(1):34. doi: 10.1186/s12989-017-0215-3
    Read this paper
  • Luo L, Guo K, Fan W, et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med. 2017,13(2):645-650. doi: 10.3892/etm.2016.4016
    Read this paper

Datasheet

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Figure 1. Western blot analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (A02128). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95/DLG4 antigen affinity purified polyclonal antibody (Catalog # A02128) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95/DLG4 at approximately 99KD. The expected band size for PSD95/DLG4 is at 99KD.
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