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Western Blot analysis of 293 cells using SAPAP1 Polyclonal Antibody
Western Blot analysis of 293 cells using SAPAP1 Polyclonal Antibody
Western Blot analysis of 293 cells using SAPAP1 Polyclonal Antibody

DLGAP1 Antibody

CSB-PA004048
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDLGAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DLGAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9229
  • Target name
    DLGAP1
  • Target description
    DLG associated protein 1
  • Target synonyms
    DAP-1, DAP-1-ALPHA, DAP-1-BETA, DAP1, DLGAP1A, DLGAP1B, GKAP, SAPAP1, disks large-associated protein 1, PSD-95/SAP90 binding protein 1, SAP90/PSD-95-associated protein 1, discs large homolog associated protein 1, guanylate kinase-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14490
  • Protein Name
    Disks large-associated protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DLGAP1 using anti-DLGAP1 antibody (A08230-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLGAP1 antigen affinity purified polyclonal antibody (Catalog # A08230-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLGAP1 at approximately 130 kDa. The expected band size for DLGAP1 is at 109 kDa.
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