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ELISA analysis of antigen using GTX60469 DLGAP1 antibody [3G4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
ELISA analysis of antigen using GTX60469 DLGAP1 antibody [3G4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
ELISA analysis of antigen using GTX60469 DLGAP1 antibody [3G4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng

DLGAP1 antibody [3G4]

GTX60469
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDLGAP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DLGAP1 antibody [3G4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G4
  • Conjugate
    Unconjugated
  • Gene ID9229
  • Target name
    DLGAP1
  • Target description
    DLG associated protein 1
  • Target synonyms
    DAP-1, DAP-1-ALPHA, DAP-1-BETA, DAP1, DLGAP1A, DLGAP1B, GKAP, SAPAP1, disks large-associated protein 1, PSD-95/SAP90 binding protein 1, SAP90/PSD-95-associated protein 1, discs large homolog associated protein 1, guanylate kinase-associated protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO14490
  • Protein Name
    Disks large-associated protein 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLGAP1 using anti-DLGAP1 antibody (A08230-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLGAP1 antigen affinity purified polyclonal antibody (Catalog # A08230-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLGAP1 at approximately 130 kDa. The expected band size for DLGAP1 is at 109 kDa.
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