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ProductsBack/DLK (DLK1) Mouse Monoclonal Antibody [Clone ID: 3A10]
Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)
Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)
Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)

DLK (DLK1) Mouse Monoclonal Antibody [Clone ID: 3A10]

AM06690SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetDLK1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    DLK (DLK1) Mouse Monoclonal Antibody [Clone ID: 3A10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A10
  • Gene ID8788
  • Target name
    DLK1
  • Target description
    delta like non-canonical Notch ligand 1
  • Target synonyms
    DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2, protein delta homolog 1, delta-like 1 homolog, fetal antigen 1, preadipocyte factor 1, secredeltin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP80370
  • Protein Name
    Protein delta homolog 1
  • Scientific Description
    DLK (DLK1) mouse monoclonal antibody, clone 3A10, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLK-1/DLK1 antigen affinity purified polyclonal antibody (Catalog # A00513-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLK-1/DLK1 at approximately 50 kDa. The expected band size for DLK-1/DLK1 is at 50 kDa.
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