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ProductsBack/DLL4 antibody [28V45]

DLL4 antibody [28V45]

GTX52972
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetDLL4
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Overview

  • Supplier
    GeneTex
  • Product Name
    DLL4 antibody [28V45]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    28V45
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID54567
  • Target name
    DLL4
  • Target description
    delta like canonical Notch ligand 4
  • Target synonyms
    AOS6; delta 4; delta ligand 4; delta4; delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; drosophila Delta homolog 4; hdelta2; notch ligand delta-2; notch ligand DLL4
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ9NR61
  • Protein Name
    Delta-like protein 4
  • Scientific Description
    This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.
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