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DRP1 Antibody(Rabbit)

HY-P80644
MedChem Express
TargetDNM1L
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DRP1 Antibody(Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10059
  • Target name
    DNM1L
  • Target description
    dynamin 1 like
  • Target synonyms
    DLP1; Dnm1p/Vps1p-like protein; DRP1; DVLP; DYMPLE; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; dynamin-related protein 1; EMPF; EMPF1; HDYNIV; OPA5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    DRP1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DRP1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.
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