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ProductsBack/DNM1L Antibody
Western Blot analysis of HELA cells using DRP1 Polyclonal Antibody
Western Blot analysis of HELA cells using DRP1 Polyclonal Antibody
Western Blot analysis of HELA cells using DRP1 Polyclonal Antibody

DNM1L Antibody

CSB-PA002203
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDNM1L
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Overview

  • Supplier
    Cusabio
  • Product Name
    DNM1L Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10059
  • Target name
    DNM1L
  • Target description
    dynamin 1 like
  • Target synonyms
    DLP1; Dnm1p/Vps1p-like protein; DRP1; DVLP; DYMPLE; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; dynamin-related protein 1; EMPF; EMPF1; HDYNIV; OPA5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00429
  • Protein Name
    Dynamin-1-like protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.
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