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ProductsBack/DNA ligase III antibody [C2C3], C-term

DNA ligase III antibody [C2C3], C-term

GTX103197
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetLIG3
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Overview

  • Supplier
    GeneTex
  • Product Name
    DNA ligase III antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.15 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3980
  • Target name
    LIG3
  • Target description
    DNA ligase 3
  • Target synonyms
    DNA ligase 3; LIG2; LIG3alpha; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49916
  • Protein Name
    DNA ligase 3
  • Scientific Description
    This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Interaction with OGG1 is required for efficient recruitment of XRCC1 to base excision repair and maintenance of genetic stability after exposure to oxidative stress. Campalans A et al., 2015 May, Mol Cell Biol
    Read more
  • DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair. Abdou I et al., 2015 Jan, Nucleic Acids Res
    Read more
  • Lobarstin enhances chemosensitivity in human glioblastoma T98G cells. Kim S et al., 2013 Dec, Anticancer Res
    Read more
  • Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells. Truong LN et al., 2013 May 7, Proc Natl Acad Sci U S A
    Read more

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Figure 1. Western blot analysis of DNA Ligase III/LIG3 using anti-DNA Ligase III/LIG3 antibody (A02741-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Ligase III/LIG3 antigen affinity purified polyclonal antibody (Catalog # A02741-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Ligase III/LIG3 at approximately 113 kDa. The expected band size for DNA Ligase III/LIG3 is at 113 kDa.
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