![HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638333/GTX638333_T-44977_20230317_WB_Fraction_23032022_926.webp)
HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DNA ligase III antibody [HL2280]
GTX638333
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLIG3
Overview
- SupplierGeneTex
- Product NameDNA ligase III antibody [HL2280]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2280
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3980
- Target nameLIG3
- Target descriptionDNA ligase 3
- Target synonymsLIG2, LIG3alpha, MTDPS20, DNA ligase 3, ligase II, DNA, ATP-dependent, ligase III, DNA, ATP-dependent, polydeoxyribonucleotide synthase [ATP] 3
- HostRabbit
- IsotypeIgG
- Protein IDP49916
- Protein NameDNA ligase 3
- Scientific DescriptionThis gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638333/GTX638333_T-44977_20230317_WB_M_R_23032022_493.webp)
![DNA ligase III antibody [HL2280] detects DNA ligase III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min DNA ligase III antibody [HL2280] detects DNA ligase III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638333/GTX638333_T-44977_20230407_IHC-P_multiple_M_23041719_757.webp)
![DNA ligase III antibody [HL2280] detects DNA ligase III protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. DNA ligase III antibody [HL2280] detects DNA ligase III protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638333/GTX638333_T-44977_20230421_ICC_IF_23050918_362.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638333/GTX638333_T-44977_20241213_WB_shRNA_watermark_24121918_261.webp)






![HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [C2C3], C-term (GTX103197) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103197/GTX103197_44349_20210702_WB_Fraction_w_23060119_184.webp)