DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_T-44676_20220701_WB_M_R_22070501_945.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_44739_20220715_WB_22072519_574.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_44739_20221216_WB_Z_22122018_152.webp "Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_44739_20221227_IHC-P_multiple_R_22122821_598.webp "DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_44739_20221230_ICC_IF_22122901_619.webp "DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_T-44676_20220610_WB_KO_watermark_w_23061202_557.webp "Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636986/GTX636986_44739_20250815_WB_medaka_25082121_639.webp "Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNA polymerase beta antibody [HL1512]
GTX636986
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetPOLB
Overview
- SupplierGeneTex
- Product NameDNA polymerase beta antibody [HL1512]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1512
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5423
- Target namePOLB
- Target descriptionDNA polymerase beta
- Target synonymsDNA polymerase beta, 5'-dRP lyase, 5'-deoxyribose-phosphate lyase, AP lyase, DNA pol beta, DNA polymerase beta subunit, polymerase (DNA directed), beta, polymerase (DNA) beta
- HostRabbit
- IsotypeIgG
- Protein IDP06746
- Protein NameDNA polymerase beta
- Scientific DescriptionThe protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![DNA polymerase beta antibody [HL1525] detects DNA polymerase beta protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1525] (GTX636999) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636999/GTX636999_44830_20221104_ICC_IF_treatment_Etoposide_22112219_526.webp)
Product group Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Zebra Fish
TargetPOLB
- SizePrice