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ProductsBack/POLB Polyclonal Antibody

POLB Polyclonal Antibody

CAC13873
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPOLB
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Overview

  • Supplier
    Biomatik
  • Product Name
    POLB Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5423
  • Target name
    POLB
  • Target description
    DNA polymerase beta
  • Target synonyms
    DNA polymerase beta, 5'-dRP lyase, 5'-deoxyribose-phosphate lyase, AP lyase, DNA pol beta, DNA polymerase beta subunit, polymerase (DNA directed), beta, polymerase (DNA) beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06746
  • Protein Name
    DNA polymerase beta
  • Scientific Description
    The POLB Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLB using anti-POLB antibody (PB9812). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLB antigen affinity purified polyclonal antibody (Catalog # PB9812) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLB at approximately 39 kDa. The expected band size for POLB is at 39 kDa.
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