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Western Blot analysis of K562 cells using DNA pol beta Polyclonal Antibody
Western Blot analysis of K562 cells using DNA pol beta Polyclonal Antibody
Western Blot analysis of K562 cells using DNA pol beta Polyclonal Antibody

POLB Antibody

CSB-PA002173
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLB
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5423
  • Target name
    POLB
  • Target description
    DNA polymerase beta
  • Target synonyms
    DNA polymerase beta, 5'-dRP lyase, 5'-deoxyribose-phosphate lyase, AP lyase, DNA pol beta, DNA polymerase beta subunit, polymerase (DNA directed), beta, polymerase (DNA) beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06746
  • Protein Name
    DNA polymerase beta
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLB using anti-POLB antibody (PB9812). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLB antigen affinity purified polyclonal antibody (Catalog # PB9812) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLB at approximately 39 kDa. The expected band size for POLB is at 39 kDa.
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