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ProductsBack/DNA polymerase iota antibody [N3C2], Internal
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA polymerase iota antibody [N3C2], Internal (GTX112583) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA polymerase iota antibody [N3C2], Internal (GTX112583) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA polymerase iota antibody [N3C2], Internal (GTX112583) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

DNA polymerase iota antibody [N3C2], Internal

GTX112583
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLI
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Overview

  • Supplier
    GeneTex
  • Product Name
    DNA polymerase iota antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.34 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11201
  • Target name
    POLI
  • Target description
    DNA polymerase iota
  • Target synonyms
    RAD30B, RAD3OB, eta2, DNA polymerase iota, RAD30 homolog B, polymerase (DNA directed) iota, polymerase (DNA) iota, polymerase (DNA-directed), iota
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNA4
  • Protein Name
    DNA polymerase iota
  • Scientific Description
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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