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ProductsBack/DNAJA2 Mouse Monoclonal Antibody [Clone ID: OTI3A10]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and DNAJA2-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2 monoclonal antibody TA501685 (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and DNAJA2-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2 monoclonal antibody TA501685 (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and DNAJA2-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2 monoclonal antibody TA501685 (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

DNAJA2 Mouse Monoclonal Antibody [Clone ID: OTI3A10]

CF501685
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetDNAJA2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    DNAJA2 Mouse Monoclonal Antibody [Clone ID: OTI3A10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3A10
  • Gene ID10294
  • Target name
    DNAJA2
  • Target description
    DnaJ heat shock protein family (Hsp40) member A2
  • Target synonyms
    CPR3, DJ3, DJA2, DNAJ, DNJ3, HIRIP4, PRO3015, RDJ2, dnaJ homolog subfamily A member 2, DnaJ (Hsp40) homolog, subfamily A, member 2, HIRA interacting protein 4, cell cycle progression 3 protein, cell cycle progression restoration gene 3 protein, renal carcinoma antigen NY-REN-14
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO60884
  • Protein Name
    DnaJ homolog subfamily A member 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) DNAJA2 mouse monoclonal antibody, clone OTI3A10 (formerly 3A10)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2 antigen affinity purified polyclonal antibody (Catalog # A08918-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJA2 at approximately 46KD. The expected band size for DNAJA2 is at 46KD.
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