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Western Blot analysis of COLO205 cells using HSP40 Polyclonal Antibody
Western Blot analysis of COLO205 cells using HSP40 Polyclonal Antibody
Western Blot analysis of COLO205 cells using HSP40 Polyclonal Antibody

DNAJB1 Antibody

CSB-PA002989
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDNAJB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DNAJB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3337
  • Target name
    DNAJB1
  • Target description
    DnaJ heat shock protein family (Hsp40) member B1
  • Target synonyms
    HSPF1, Hdj1, Hsp40, RSPH16B, Sis1, dnaJ homolog subfamily B member 1, DnaJ (Hsp40) homolog, subfamily B, member 1, dnaJ protein homolog 1, heat shock 40 kDa protein 1, human DnaJ protein 1, radial spoke 16 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25685
  • Protein Name
    DnaJ homolog subfamily B member 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.
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Lane 1: 293T cell lysates; Lane 2: MCF-7 lysates; Lane 3: BxPC-3 cell lysates; Lane 4: BT-474 cell lysates; Lane 5: SW620 cell lysates; Lane 6: K562 cell lysates; Lane 7: Mouse testis lysates; Lane 8: Mouse spleen lysates; Lane 9: Mouse thymus lysates probed with DNAJB1 Polyclonal Antibody, Unconjugated (bs-55055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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