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Hsp40 Antibody (YA731, Mouse)

HY-P80710
MedChem Express
TargetDNAJB1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Hsp40 Antibody (YA731, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3337
  • Target name
    DNAJB1
  • Target description
    DnaJ heat shock protein family (Hsp40) member B1
  • Target synonyms
    HSPF1, Hdj1, Hsp40, RSPH16B, Sis1, dnaJ homolog subfamily B member 1, DnaJ (Hsp40) homolog, subfamily B, member 1, dnaJ protein homolog 1, heat shock 40 kDa protein 1, human DnaJ protein 1, radial spoke 16 homolog B
  • Host
    Mouse
  • Isotype
    IgG
  • Scientific Description
    Hsp40 Antibody (YA731) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp40.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.
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