Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2553] (GTX638925) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Dopamine Receptor D1 antibody [HL2553] (GTX638925) detects Dopamine Receptor D1 protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Dopamine Receptor D1 antibody [HL2553] (GTX638925) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638925/GTX638925_45313_20251017_FCM_25112100_105.webp "Dopamine Receptor D1 antibody [HL2553] (GTX638925) detects Dopamine Receptor D1 protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Dopamine Receptor D1 antibody [HL2553] (GTX638925) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.")
Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2553] (GTX638925) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Dopamine Receptor D1 antibody [HL2553]
GTX638925
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetDRD1
Overview
- SupplierGeneTex
- Product NameDopamine Receptor D1 antibody [HL2553]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2553
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1812
- Target nameDRD1
- Target descriptiondopamine receptor D1
- Target synonymsD1R, DADR, DRD1A, D(1A) dopamine receptor, dopamine D1 receptor
- HostRabbit
- IsotypeIgG
- Protein IDP21728
- Protein NameD(1A) dopamine receptor
- Scientific DescriptionThis gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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![Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639344/GTX639344_T-45222_20240216_WB_multiple_B_24021917_587.webp)
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![Dopamine Receptor D1 antibody [HL2927] detects Dopamine Receptor D1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [HL2927] (GTX640270) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640270/GTX640270_T-45390_20240510_ICC_IF_B_24070822_338.webp)
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