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Dopamine Receptor D5 antibody

GTX77969
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Other Species
TargetDRD5
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Overview

  • Supplier
    GeneTex
  • Product Name
    Dopamine Receptor D5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 3.4 microg/ml. IHC-P: 3.4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1816
  • Target name
    DRD5
  • Target description
    dopamine receptor D5
  • Target synonyms
    DBDR, DRD1B, DRD1L2, D(1B) dopamine receptor, D1beta dopamine receptor, d(5) dopamine receptor, dopamine D5 receptor, dopamine receptor D1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21918
  • Protein Name
    D(1B) dopamine receptor
  • Scientific Description
    This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat, Other Species
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kajiwara M, Ban T, Matsubara K, et al. Urinary Dopamine as a Potential Index of the Transport Activity of Multidrug and Toxin Extrusion in the Kidney. Int J Mol Sci. 2016,17(8). doi: 10.3390/ijms17081228
    Read this paper
  • Arnaldo FB, Villar VA, Konkalmatt PR, et al. D1-like dopamine receptors downregulate Na+-K+-ATPase activity and increase cAMP production in the posterior gills of the blue crab Callinectes sapidus. Am J Physiol Regul Integr Comp Physiol. 2014,307(6):R634-42. doi: 10.1152/ajpregu.00555.2013
    Read this paper

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Figure 1. Western blot analysis of DRD5 using anti-DRD5 antibody (A03010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD5 antigen affinity purified polyclonal antibody (Catalog # A03010-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRD5 at approximately 58 kDa. The expected band size for DRD5 is at 53 kDa.
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