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Western blot All lanes: DRD5 antibody at 2microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: DRD5 antibody at 2microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: DRD5 antibody at 2microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa

DRD5 Antibody

CSB-PA007182EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDRD5
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Overview

  • Supplier
    Cusabio
  • Product Name
    DRD5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1816
  • Target name
    DRD5
  • Target description
    dopamine receptor D5
  • Target synonyms
    DBDR, DRD1B, DRD1L2, D(1B) dopamine receptor, D1beta dopamine receptor, d(5) dopamine receptor, dopamine D5 receptor, dopamine receptor D1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21918
  • Protein Name
    D(1B) dopamine receptor
  • Scientific Description
    Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DRD5 using anti-DRD5 antibody (A03010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD5 antigen affinity purified polyclonal antibody (Catalog # A03010-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRD5 at approximately 58 kDa. The expected band size for DRD5 is at 53 kDa.
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