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PDP2 Antibody

CAB17190
Assay Genie
TargetDRD5
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PDP2 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1816
  • Target name
    DRD5
  • Target description
    dopamine receptor D5
  • Target synonyms
    D(1B) dopamine receptor; d(5) dopamine receptor; D1beta dopamine receptor; DBDR; dopamine D5 receptor; dopamine receptor D1B; DRD1B; DRD1L2
  • Host
    Rabbit
  • Protein IDP21918
  • Protein Name
    D(1B) dopamine receptor
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DRD5 using anti-DRD5 antibody (A03010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD5 antigen affinity purified polyclonal antibody (Catalog # A03010-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRD5 at approximately 58 kDa. The expected band size for DRD5 is at 53 kDa.
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