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Western Blot analysis of Jurkat cells using MKP-1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MKP-1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MKP-1 Polyclonal Antibody

DUSP1 Antibody

CSB-PA003245
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDUSP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DUSP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1843
  • Target name
    DUSP1
  • Target description
    dual specificity phosphatase 1
  • Target synonyms
    CL 100; CL100; dual specificity protein phosphatase 1; dual specificity protein phosphatase hVH1; HVH1; MAP kinase phosphatase 1; mitogen-activated protein kinase phosphatase 1; MKP1; MKP-1; protein-tyrosine phosphatase CL100; PTPN10; serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28562
  • Protein Name
    Dual specificity protein phosphatase 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (A02276-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (Catalog # A02276-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40KD. The expected band size for MKP-1/DUSP1 is at 40KD.
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