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Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.

MKP1 Polyclonal Antibody

BS-1851R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDUSP1
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Overview

  • Supplier
    Bioss
  • Product Name
    MKP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1843
  • Target name
    DUSP1
  • Target description
    dual specificity phosphatase 1
  • Target synonyms
    CL100, HVH1, MKP-1, MKP1, PTPN10, dual specificity protein phosphatase 1, CL 100, MAP kinase phosphatase 1, dual specificity protein phosphatase hVH1, mitogen-activated protein kinase phosphatase 1, protein-tyrosine phosphatase CL100, serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28562
  • Protein Name
    Dual specificity protein phosphatase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sha J, Feng X, Chen Y, et al. Dexmedetomidine improves acute stress-induced liver injury in rats by regulating MKP-1, inhibiting NF-κB pathway and cell apoptosis. J Cell Physiol. 2019,234(8):14068-14078. doi: 10.1002/jcp.28096
    Read this paper
  • Sha J, Zhang H, Zhao Y, et al. Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor. Toxicol Appl Pharmacol. 2019,364:144-152. doi: 10.1016/j.taap.2018.12.017
    Read this paper
  • Kouam AF, Yuan F, Njayou FN, et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Front Pharmacol. 2017,8:653. doi: 10.3389/fphar.2017.00653
    Read this paper
  • Kato H, Naiki-Ito A, Naiki T, et al. Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis. Oncotarget. 2016,7(2):2009-21. doi: 10.18632/oncotarget.6511
    Read this paper

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Figure 1. Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (A02276-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (Catalog # A02276-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40KD. The expected band size for MKP-1/DUSP1 is at 40KD.
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