More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MKP1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.

MKP1 Polyclonal Antibody

BS-1851R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
TargetDUSP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    MKP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1843
  • Target name
    DUSP1
  • Target description
    dual specificity phosphatase 1
  • Target synonyms
    CL100, HVH1, MKP-1, MKP1, PTPN10, dual specificity protein phosphatase 1, CL 100, MAP kinase phosphatase 1, dual specificity protein phosphatase hVH1, mitogen-activated protein kinase phosphatase 1, protein-tyrosine phosphatase CL100, serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28562
  • Protein Name
    Dual specificity protein phosphatase 1
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Dexmedetomidine improves acute stress-induced liver injury in rats by regulating MKP-1, inhibiting NF-kappaB pathway and cell apoptosis. Sha J et al., 2019 Aug, J Cell Physiol
    Read this paper
  • Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3beta/MKP-1/Nrf2 pathway activity via the alpha2 adrenergic receptor. Sha J et al., 2019 Feb 1, Toxicol Appl Pharmacol
    Read this paper
  • Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Kouam AF et al., 2017, Front Pharmacol
    Read this paper
  • Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis. Kato H et al., 2016 Jan 12, Oncotarget
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
DUSP1/MKP1 AntibodyCAB2919
ReactivityHuman
TargetDUSP1
  • SizePrice
Western Blot analysis of Jurkat cells using MKP-1 Polyclonal Antibody
Product group Antibodies
Cusabio
DUSP1 AntibodyCSB-PA003245
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDUSP1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-DUSP1 Antibody144-02919
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetDUSP1
  • SizePrice
Anti-DUSP1 Antibody
Product group Antibodies
Antibodies.com
Anti-DUSP1 AntibodyA43339
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Figure 1. Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (A02276-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (Catalog # A02276-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40KD. The expected band size for MKP-1/DUSP1 is at 40KD.
Product group Antibodies
Boster Bio
Anti-MKP-1/DUSP1 Antibody Picoband(r)A02276-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetDUSP1
  • SizePrice
Product group Antibodies
MedChem Express
DUSP1 Antibody(Rabbit)HY-P80645
TargetDUSP1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-DUSP1EB05197
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Rat
TargetDUSP1
  • SizePrice
Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & cytosol.
Product group Antibodies
Atlas Antibodies
Anti-DUSP1 AntibodyHPA069577
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetDUSP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DUSP1 / MKP1 AntibodyLS-C402268
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDUSP1
  • SizePrice
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with DUSP1 antibody [N3C3] (GTX101229) diluted at 1:1000 and competitor's antibody (sc-1199) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
DUSP1 antibodyGTX101229
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetDUSP1
  • SizePrice