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E2F-1 Antibody (YA471, Rabbit)

HY-P80491
MedChem Express
TargetE2F1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    E2F-1 Antibody (YA471, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1869
  • Target name
    E2F1
  • Target description
    E2F transcription factor 1
  • Target synonyms
    E2F-1, RBAP1, RBBP3, RBP3, transcription factor E2F1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, retinoblastoma-binding protein 3 (E2F-like)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    E2F-1 Antibody (YA471) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E2F-1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of E2F1 using anti-E2F1 antibody (A00257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F1 antigen affinity purified polyclonal antibody (Catalog # A00257-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F1 at approximately 70 kDa. The expected band size for E2F1 is at 47 kDa.
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