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Mouse skeleton lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse skeleton lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse skeleton lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

E2F1 Polyclonal Antibody

BS-0599R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetE2F1
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Overview

  • Supplier
    Bioss
  • Product Name
    E2F1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1869
  • Target name
    E2F1
  • Target description
    E2F transcription factor 1
  • Target synonyms
    E2F-1, RBAP1, RBBP3, RBP3, transcription factor E2F1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, retinoblastoma-binding protein 3 (E2F-like)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01094
  • Protein Name
    Transcription factor E2F1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Muhammad T, Sakhawat A, Khan AA, et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020,146(4):861-874. doi: 10.1007/s00432-020-03157-2
    Read this paper
  • Liu J, Yang M, Jing L, et al. Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice. Environ Sci Pollut Res Int. 2018,25(4):3423-3434. doi: 10.1007/s11356-017-0724-5
    Read this paper
  • Sopariwala DH, Yadav V, Badin PM, et al. Long-term PGC1β overexpression leads to apoptosis, autophagy and muscle wasting. Sci Rep. 2017,7(1):10237. doi: 10.1038/s41598-017-10238-9
    Read this paper

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Figure 1. Western blot analysis of E2F1 using anti-E2F1 antibody (A00257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F1 antigen affinity purified polyclonal antibody (Catalog # A00257-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F1 at approximately 70 kDa. The expected band size for E2F1 is at 47 kDa.
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