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E2F4/E2F5 Antibody

PACO00732
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetE2F4
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Overview

  • Supplier
    Assay Genie
  • Product Name
    E2F4/E2F5 Antibody (PACO00732)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1874
  • Target name
    E2F4
  • Target description
    E2F transcription factor 4
  • Target synonyms
    E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; transcription factor E2F4
  • Host
    Rabbit
  • Protein IDQ15329
  • Protein Name
    Transcription factor E2F5
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human, Mouse, Rat, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of E2F4 using anti-E2F4 antibody (PB10057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: U20S whole cell lysates, Lane 3: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F4 antigen affinity purified polyclonal antibody (Catalog # PB10057) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F4 at approximately 44 kDa, 60 kDa. The expected band size for E2F4 is at 44 kDa.
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