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EAAC1 Antibody (OASE00254)

OASE00254
Aviva Systems Biology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSLC1A1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    EAAC1 Antibody (OASE00254)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S180-41
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6505
  • Target name
    SLC1A1
  • Target description
    solute carrier family 1 member 1
  • Target synonyms
    DCBXA, EAAC1, EAAT3, SCZD18, hEAAC1, excitatory amino acid transporter 3, excitatory amino acid carrier 1, neuronal and epithelial glutamate transporter, sodium-dependent glutamate/aspartate transporter 3, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EAAT3/SLC1A1 using anti-EAAT3/SLC1A1 antibody (A02367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT3/SLC1A1 antigen affinity purified polyclonal antibody (Catalog # A02367-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT3/SLC1A1 at approximately 70KD. The expected band size for EAAT3/SLC1A1 is at 70KD.
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