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EBAG9 / RCAS1 Antibody

LS-C490227
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEBAG9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EBAG9 / RCAS1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9166
  • Target name
    EBAG9
  • Target description
    estrogen receptor binding site associated antigen 9
  • Target synonyms
    cancer-associated surface antigen RCAS1; EB9; estrogen receptor-binding fragment-associated gene 9 protein; PDAF; receptor-binding cancer antigen expressed on SiSo cells
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBAG9 using anti-EBAG9 antibody (PB9553). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: 22RV1 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug, Lane 5: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBAG9 antigen affinity purified polyclonal antibody (Catalog # PB9553) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBAG9 at approximately 34 kDa. The expected band size for EBAG9 is at 34 kDa.
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