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ProductsBack/Goat anti-EBAG9 / RCAS1

Goat anti-EBAG9 / RCAS1

EB07292
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEBAG9
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-EBAG9 / RCAS1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9166
  • Target name
    EBAG9
  • Target description
    estrogen receptor binding site associated antigen 9
  • Target synonyms
    EB9, PDAF, receptor-binding cancer antigen expressed on SiSo cells, cancer-associated surface antigen RCAS1, estrogen receptor-binding fragment-associated gene 9 protein, receptor-binding cancer antigen expressed on SiSo cells 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004206.1. GeneIDs all Nonhuman: 55960 (mouse); 299864 (rat);. Purification: Antigen affinity purified. Names and symbols: EBAG9; RCAS1; estrogen receptor binding site associated, antigen, 9 ; HGNC:3123; EB9; PDAF; cancer associated surface antigen; estrogen receptor binding site associated antigen 9
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBAG9 using anti-EBAG9 antibody (PB9553). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: 22RV1 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug, Lane 5: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBAG9 antigen affinity purified polyclonal antibody (Catalog # PB9553) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBAG9 at approximately 34 kDa. The expected band size for EBAG9 is at 34 kDa.
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