Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RCAS1/EBAG9 Polyclonal Antibody, Unconjugated (bs-10982R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RCAS1/EBAG9 Polyclonal Antibody, Unconjugated (bs-10982R) at 1:500 overnight at 4°C, DAB staining.
RCAS1/EBAG9 Polyclonal Antibody
BS-10982R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Human, Mouse, Porcine, Rabbit
TargetEBAG9
Overview
- SupplierBioss Antibodies
- Product NameRCAS1/EBAG9 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID9166
- Target nameEBAG9
- Target descriptionestrogen receptor binding site associated antigen 9
- Target synonymscancer-associated surface antigen RCAS1; EB9; estrogen receptor-binding fragment-associated gene 9 protein; PDAF; receptor-binding cancer antigen expressed on SiSo cells
- HostRabbit
- IsotypeIgG
- Protein IDO00559
- Protein NameReceptor-binding cancer antigen expressed on SiSo cells
- ReactivityBovine, Chicken, Human, Mouse, Porcine, Rabbit
- Storage Instruction-20°C
- UNSPSC12352203