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ProductsBack/EBP1 Antibody (YA2509, Rabbit)

EBP1 Antibody (YA2509, Rabbit)

HY-P82764
MedChem Express
TargetPA2G4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    EBP1 Antibody (YA2509, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5036
  • Target name
    PA2G4
  • Target description
    proliferation-associated 2G4
  • Target synonyms
    EBP1, HG4-1, ITAF45, p38-2G4, proliferation-associated protein 2G4, ErbB-3 binding protein 1, ErbB-3 receptor binding protein, ErbB3-binding protein Ebp1, IRES-specific cellular transacting factor 45, cell cycle protein p38-2G4 homolog, erbB3-binding protein 1, proliferation-associated 2G4, 38kD, proliferation-associated 2G4, 38kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    EBP1 Antibody (YA2509) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2509), targeting EBP1, with a predicted molecular weight of 44 kDa (observed band size: 48 kDa). EBP1 Antibody (YA2509) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EBP1 using anti-EBP1 antibody (A02791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat liver tissue lysates, lane 2: NIH3T3 whole cell lysates, lane 3: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBP1 antigen affinity purified polyclonal antibody (Catalog # A02791) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBP1 at approximately 43KD. The expected band size for EBP1 is at 43KD.
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