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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ECM1 antibody at 4.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 47, 20, 64 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ECM1 antibody at 4.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 47, 20, 64 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ECM1 antibody at 4.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 47, 20, 64 kDa Observed band size: 47 kDa

ECM1 Antibody

CSB-PA007383LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetECM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ECM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1893
  • Target name
    ECM1
  • Target description
    extracellular matrix protein 1
  • Target synonyms
    extracellular matrix protein 1; secretory component p85; testicular tissue protein Li 61; URBWD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16610
  • Protein Name
    Extracellular matrix protein 1
  • Scientific Description
    Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.
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