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Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.

ECM1 Polyclonal Antibody

BS-0776R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetECM1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ECM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1893
  • Target name
    ECM1
  • Target description
    extracellular matrix protein 1
  • Target synonyms
    extracellular matrix protein 1; secretory component p85; testicular tissue protein Li 61; URBWD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16610
  • Protein Name
    Extracellular matrix protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Common expression of stemness molecular markers and early cardiac transcription factors in human Whartons jelly-derived mesenchymal stem cells and embryonic stem cells. Gao LR et al., 2013, Cell Transplant
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