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Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.

ECM1 Polyclonal Antibody

BS-0776R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetECM1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ECM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1893
  • Target name
    ECM1
  • Target description
    extracellular matrix protein 1
  • Target synonyms
    extracellular matrix protein 1; secretory component p85; testicular tissue protein Li 61; URBWD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16610
  • Protein Name
    Extracellular matrix protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Common expression of stemness molecular markers and early cardiac transcription factors in human Whartons jelly-derived mesenchymal stem cells and embryonic stem cells. Gao LR et al., 2013, Cell Transplant
    Read more

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Figure 1. Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.
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