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ECM1 Antibody, FITC conjugated

MBS7107853
MyBioSource
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Product group Antibodies
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0.05 mg
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Overview

  • Supplier
    MyBioSource
  • Product Name
    ECM1 Antibody, FITC conjugated
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Conjugate
    FITC
  • Estimated Purity
    >95%, Protein G purified
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.
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