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WB analysis of UV-treated HepG2 (A), mouse brain (B) whole cell lysates using GTX55030 EDG1 (phospho Thr236) antibody.
WB analysis of UV-treated HepG2 (A), mouse brain (B) whole cell lysates using GTX55030 EDG1 (phospho Thr236) antibody.
WB analysis of UV-treated HepG2 (A), mouse brain (B) whole cell lysates using GTX55030 EDG1 (phospho Thr236) antibody.

EDG1 (phospho Thr236) antibody

GTX55030
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetS1PR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EDG1 (phospho Thr236) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1901
  • Target name
    S1PR1
  • Target description
    sphingosine-1-phosphate receptor 1
  • Target synonyms
    CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; S1P receptor 1; S1P receptor Edg-1; S1P1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21453
  • Protein Name
    Sphingosine 1-phosphate receptor 1
  • Scientific Description
    The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDG1 using anti-EDG1 antibody (M01502). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG1 antigen affinity purified monoclonal antibody (Catalog # M01502) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG1 at approximately 43 kDa. The expected band size for EDG1 is at 43 kDa.
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