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Western Blot analysis of K562 cells using EEA1 Polyclonal Antibody
Western Blot analysis of K562 cells using EEA1 Polyclonal Antibody
Western Blot analysis of K562 cells using EEA1 Polyclonal Antibody

EEA1 Antibody

CSB-PA004632
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetEEA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EEA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8411
  • Target name
    EEA1
  • Target description
    early endosome antigen 1
  • Target synonyms
    MST105, MSTP105, ZFYVE2, early endosome antigen 1, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15075
  • Protein Name
    Early endosome antigen 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: humna K562 whole cell lysates, Lane 6: humna MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (Catalog # A02296-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
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