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WB analysis of various sample lysates using GTX33168 EED antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX33168 EED antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX33168 EED antibody. Dilution : 1:1000 Loading : 25μg per lane

EED antibody

GTX33168
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetEED
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Overview

  • Supplier
    GeneTex
  • Product Name
    EED antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8726
  • Target name
    EED
  • Target description
    embryonic ectoderm development
  • Target synonyms
    COGIS, HEED, WAIT1, polycomb protein EED, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75530
  • Protein Name
    Polycomb protein EED
  • Scientific Description
    This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. Su D et al., 2021 May 7, Nucleic Acids Res
    Read this paper

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Figure 1. Western blot analysis of EED using anti-EED antibody (A01345-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (Catalog # A01345-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EED at approximately 70 kDa. The expected band size for EED is at 50 kDa.
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