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Western Blot analysis of HeLa cells using eEF2K Polyclonal Antibody
Western Blot analysis of HeLa cells using eEF2K Polyclonal Antibody
Western Blot analysis of HeLa cells using eEF2K Polyclonal Antibody

EEF2K Antibody

CSB-PA002258
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetEEF2K
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Overview

  • Supplier
    Cusabio
  • Product Name
    EEF2K Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29904
  • Target name
    EEF2K
  • Target description
    eukaryotic elongation factor 2 kinase
  • Target synonyms
    CaMKIII, HSU93850, eEF-2K, eukaryotic elongation factor 2 kinase, alternative protein EEF2K, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, calmodulin-dependent protein kinase III, eEF-2 kinase, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00418
  • Protein Name
    Eukaryotic elongation factor 2 kinase
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EEF2K using anti-EEF2K antibody (A02277-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2K antigen affinity purified polyclonal antibody (Catalog # A02277-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEF2K at approximately 82 kDa. The expected band size for EEF2K is at 82 kDa.
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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
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