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ProductsBack/eEF2K antibody [N1N3]
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) 7.5% SDS PAGE GTX111496 diluted at 1:5000
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) 7.5% SDS PAGE GTX111496 diluted at 1:5000
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) 7.5% SDS PAGE GTX111496 diluted at 1:5000

eEF2K antibody [N1N3]

GTX111496
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEEF2K
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Overview

  • Supplier
    GeneTex
  • Product Name
    eEF2K antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29904
  • Target name
    EEF2K
  • Target description
    eukaryotic elongation factor 2 kinase
  • Target synonyms
    CaMKIII, HSU93850, eEF-2K, eukaryotic elongation factor 2 kinase, alternative protein EEF2K, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, calmodulin-dependent protein kinase III, eEF-2 kinase, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00418
  • Protein Name
    Eukaryotic elongation factor 2 kinase
  • Scientific Description
    This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liong S, Lappas M. Markers of protein synthesis are increased in fetal membranes and myometrium after human labour and delivery. Reprod Fertil Dev. 2018,30(2):313-329. doi: 10.1071/RD17081
    Read this paper
  • Usui T, Nijima R, Sakatsume T, et al. Eukaryotic elongation factor 2 kinase controls proliferation and migration of vascular smooth muscle cells. Acta Physiol (Oxf). 2015,213(2):472-80. doi: 10.1111/apha.12354
    Read this paper

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Figure 1. Western blot analysis of EEF2K using anti-EEF2K antibody (A02277-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2K antigen affinity purified polyclonal antibody (Catalog # A02277-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEF2K at approximately 82 kDa. The expected band size for EEF2K is at 82 kDa.
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